The bus was traveling from Nablus, when it was attacked by the settlers from a hill near Burqa village, one of those injured, Raed Ewais, said.
Settlers threw stones, damaging the bus and injuring passengers, witnesses said.
The incident came after settlers gathered on the same road on Thursday night near Sinjil, throwing stones at passing vehicles.
Locals said a number of vehicles were damaged by rocks. The group caused the road to close for over an hour, they added.
A Palestinian Authority official on Monday warned Palestinians to be cautious of settler attacks ahead of a planned evacuation of an illegal Israeli outpost near Ramallah.
The hilltop settler outpost Migron is set to be evacuated this Tuesday.
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